Peter Klipfel

my musings

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2020

  1. Project graveyards as guidepostsI was inspired recently to think about old, dead projects as guideposts based on another idea: reasearch graveyards
  2. How I manage my notesI have spent a long time trying to find the right practice for taking notes, and I think I finally found a method that I like
  3. Cell LysisCell lysis is the scientist name for 'breaking open a cell'. Here I go over ways of breaking open cells.
  4. Growing neurons OverviewThis is the first in a series of blog posts about what it takes to grow neuronal cultures.
  5. CryopreservationSaving cells for future use is extermely useful. In this post, I go over a basic cryopreservation technique
  6. MAP kinaseMAP kinase comes up as a canonical example in much of systems biology, and I wanted to write a short post about what it actually is
  7. Immortal cell linesImmortal cell lines are immensely useful to research. They allow us to produce fully differentiated cells in high quantities.
  8. Building proteinsOne of the foundations of biology is proteins.
  9. Lac operonI have heard a lot about the lac operon, and had never looked into it. I've taken this opportunity to explain the basics of how it can be used to select for engineered organisms
  10. DNA methylation in prokaryotesDNA methylation has profound effects in genetics which are still being studied. Methylation affects synthetic biologists by digesting transferred DNA and introducing mutations to …